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a375 ma2  (ATCC)


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    ATCC a375 ma2
    Melanoma L-EVs increase endothelial tube formation, distinct from sEVs. A , SVEC4–10 endothelial cells were incubated with no-treatment 1X PBS control, VEGF (20 ng/ml), or LOX L-EVs or sEVs, as described in the section. Cells were allowed to form tubes, and networks were imaged on an inverted microscope after 5 h. B , resulting tube networks were analyzed for the number of segments using Fiji and normalized to respective no-treatment controls among replicates. C and D , endothelial cells were incubated with L-EVs or sEVs derived from paired primary and metastatic cell lines A375P or <t>A375-MA2,</t> and the number of segments was quantified using Fiji. Data are presented as means ± SD. The p values were obtained by one-way ANOVA with Dunnett’s correction (ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001) from at least three independent experiments. L-EV, large extracellular vesicle; sEV, small extracellular vesicle; VEGF, vascular endothelial growth factor.
    A375 Ma2, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a375 ma2/product/ATCC
    Average 95 stars, based on 60 article reviews
    a375 ma2 - by Bioz Stars, 2026-03
    95/100 stars

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    1) Product Images from "Large extracellular vesicles regulate endothelial angiogenic potential via paracrine and autocrine signaling"

    Article Title: Large extracellular vesicles regulate endothelial angiogenic potential via paracrine and autocrine signaling

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111193

    Melanoma L-EVs increase endothelial tube formation, distinct from sEVs. A , SVEC4–10 endothelial cells were incubated with no-treatment 1X PBS control, VEGF (20 ng/ml), or LOX L-EVs or sEVs, as described in the section. Cells were allowed to form tubes, and networks were imaged on an inverted microscope after 5 h. B , resulting tube networks were analyzed for the number of segments using Fiji and normalized to respective no-treatment controls among replicates. C and D , endothelial cells were incubated with L-EVs or sEVs derived from paired primary and metastatic cell lines A375P or A375-MA2, and the number of segments was quantified using Fiji. Data are presented as means ± SD. The p values were obtained by one-way ANOVA with Dunnett’s correction (ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001) from at least three independent experiments. L-EV, large extracellular vesicle; sEV, small extracellular vesicle; VEGF, vascular endothelial growth factor.
    Figure Legend Snippet: Melanoma L-EVs increase endothelial tube formation, distinct from sEVs. A , SVEC4–10 endothelial cells were incubated with no-treatment 1X PBS control, VEGF (20 ng/ml), or LOX L-EVs or sEVs, as described in the section. Cells were allowed to form tubes, and networks were imaged on an inverted microscope after 5 h. B , resulting tube networks were analyzed for the number of segments using Fiji and normalized to respective no-treatment controls among replicates. C and D , endothelial cells were incubated with L-EVs or sEVs derived from paired primary and metastatic cell lines A375P or A375-MA2, and the number of segments was quantified using Fiji. Data are presented as means ± SD. The p values were obtained by one-way ANOVA with Dunnett’s correction (ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001) from at least three independent experiments. L-EV, large extracellular vesicle; sEV, small extracellular vesicle; VEGF, vascular endothelial growth factor.

    Techniques Used: Incubation, Control, Inverted Microscopy, Derivative Assay



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    a375 ma2  (ATCC)
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    ATCC a375 ma2
    Melanoma L-EVs increase endothelial tube formation, distinct from sEVs. A , SVEC4–10 endothelial cells were incubated with no-treatment 1X PBS control, VEGF (20 ng/ml), or LOX L-EVs or sEVs, as described in the section. Cells were allowed to form tubes, and networks were imaged on an inverted microscope after 5 h. B , resulting tube networks were analyzed for the number of segments using Fiji and normalized to respective no-treatment controls among replicates. C and D , endothelial cells were incubated with L-EVs or sEVs derived from paired primary and metastatic cell lines A375P or <t>A375-MA2,</t> and the number of segments was quantified using Fiji. Data are presented as means ± SD. The p values were obtained by one-way ANOVA with Dunnett’s correction (ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001) from at least three independent experiments. L-EV, large extracellular vesicle; sEV, small extracellular vesicle; VEGF, vascular endothelial growth factor.
    A375 Ma2, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a375 ma2/product/ATCC
    Average 95 stars, based on 1 article reviews
    a375 ma2 - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    a375 ma2 cells  (ATCC)
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    ATCC a375 ma2 cells
    Melanoma L-EVs increase endothelial tube formation, distinct from sEVs. A , SVEC4–10 endothelial cells were incubated with no-treatment 1X PBS control, VEGF (20 ng/ml), or LOX L-EVs or sEVs, as described in the section. Cells were allowed to form tubes, and networks were imaged on an inverted microscope after 5 h. B , resulting tube networks were analyzed for the number of segments using Fiji and normalized to respective no-treatment controls among replicates. C and D , endothelial cells were incubated with L-EVs or sEVs derived from paired primary and metastatic cell lines A375P or <t>A375-MA2,</t> and the number of segments was quantified using Fiji. Data are presented as means ± SD. The p values were obtained by one-way ANOVA with Dunnett’s correction (ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001) from at least three independent experiments. L-EV, large extracellular vesicle; sEV, small extracellular vesicle; VEGF, vascular endothelial growth factor.
    A375 Ma2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a375 ma2 cells/product/ATCC
    Average 95 stars, based on 1 article reviews
    a375 ma2 cells - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    a375 m2  (ATCC)
    95
    ATCC a375 m2
    Melanoma L-EVs increase endothelial tube formation, distinct from sEVs. A , SVEC4–10 endothelial cells were incubated with no-treatment 1X PBS control, VEGF (20 ng/ml), or LOX L-EVs or sEVs, as described in the section. Cells were allowed to form tubes, and networks were imaged on an inverted microscope after 5 h. B , resulting tube networks were analyzed for the number of segments using Fiji and normalized to respective no-treatment controls among replicates. C and D , endothelial cells were incubated with L-EVs or sEVs derived from paired primary and metastatic cell lines A375P or <t>A375-MA2,</t> and the number of segments was quantified using Fiji. Data are presented as means ± SD. The p values were obtained by one-way ANOVA with Dunnett’s correction (ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001) from at least three independent experiments. L-EV, large extracellular vesicle; sEV, small extracellular vesicle; VEGF, vascular endothelial growth factor.
    A375 M2, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a375 m2/product/ATCC
    Average 95 stars, based on 1 article reviews
    a375 m2 - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Melanoma L-EVs increase endothelial tube formation, distinct from sEVs. A , SVEC4–10 endothelial cells were incubated with no-treatment 1X PBS control, VEGF (20 ng/ml), or LOX L-EVs or sEVs, as described in the section. Cells were allowed to form tubes, and networks were imaged on an inverted microscope after 5 h. B , resulting tube networks were analyzed for the number of segments using Fiji and normalized to respective no-treatment controls among replicates. C and D , endothelial cells were incubated with L-EVs or sEVs derived from paired primary and metastatic cell lines A375P or A375-MA2, and the number of segments was quantified using Fiji. Data are presented as means ± SD. The p values were obtained by one-way ANOVA with Dunnett’s correction (ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001) from at least three independent experiments. L-EV, large extracellular vesicle; sEV, small extracellular vesicle; VEGF, vascular endothelial growth factor.

    Journal: The Journal of Biological Chemistry

    Article Title: Large extracellular vesicles regulate endothelial angiogenic potential via paracrine and autocrine signaling

    doi: 10.1016/j.jbc.2026.111193

    Figure Lengend Snippet: Melanoma L-EVs increase endothelial tube formation, distinct from sEVs. A , SVEC4–10 endothelial cells were incubated with no-treatment 1X PBS control, VEGF (20 ng/ml), or LOX L-EVs or sEVs, as described in the section. Cells were allowed to form tubes, and networks were imaged on an inverted microscope after 5 h. B , resulting tube networks were analyzed for the number of segments using Fiji and normalized to respective no-treatment controls among replicates. C and D , endothelial cells were incubated with L-EVs or sEVs derived from paired primary and metastatic cell lines A375P or A375-MA2, and the number of segments was quantified using Fiji. Data are presented as means ± SD. The p values were obtained by one-way ANOVA with Dunnett’s correction (ns, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001) from at least three independent experiments. L-EV, large extracellular vesicle; sEV, small extracellular vesicle; VEGF, vascular endothelial growth factor.

    Article Snippet: A375P (Research Resource Identifier [RRID]: CVCL_6233), A375-MA2 (RRID: CVCL_X495), MDA-MB-468 (RRID: CVCL_0419), 786-O (RRID: CVCL_1051), SVEC4–10 (RRID: CVCL_4393), HUVEC (RRID: CVCL_9Q53), and BJ Fibroblast (RRID: CVCL_3653) cell lines were purchased from American Type Culture Collection.

    Techniques: Incubation, Control, Inverted Microscopy, Derivative Assay